Unidirectional <110> bulk L-Threonine acetate (LTA) single crystal was successfully grown for the first time by Sankaranarayan–Ramasamy (SR) method. The <110> oriented plane have been placed as seed at the bottom of the ampoule and a transparent single crystal of LTA of 10 mm diameter and 50 mm length have been grown. The crystal system and lattice parameters were analyzed from the single crystal x-ray diffraction study. FT-IR and FT-Raman analyses were used to confirm the presence of the functional groups. The grown crystal was also characterized by UV-Vis-NIR absorption and micro hardness. The second harmonic generation (SHG) in the sample was confirmed by Kurtz and Perry powder technique. The laser damage threshold was also found.