Introduction: Helicobacter pylori infection is generally acquired in childhood. Algeria is a country with a high prevalence of H. pylori infection. The aim of this work is to take stock of H. pylori infection in Algerian children. Materials and Methods: The culture of 31 biopsies was performed and the sensitivity to antibiotics tested. The statuses of cagPAI and vacA were determined as well as geographical typing by Multilocus Sequence Typing (MLST). Results: Culture was positive in 12 children. One resistance to clarithromycin and one metronidazole resistance were detected. Four out of six strains possessed cagPAI and five out of six strains were vacA s2m2i2d2. The five strains tested by MLST are of the hpEurope type. Conclusion: The study reveals a high infection rate and low resistance to antibiotics and reports for the first time in Algeria a genetic typing of strains isolated in Pediatrics.