This study describe the procedure of extraction and spectroscopic analysis of Hesperidin, a naturally occurring citrus flavanone glycoside from leaves peel of three different citrus species, mandarin (C.reticulata),Clementine( C.clementina), sweet orange (C. sinensis). Hesperidin was isolated from orange peel by extracting the dry peel with petroleum ether. Spectroscopic analysis (UV, IR and NMR) confirms the structure and extent of purity of extracted Hesperidin. An improved procedure for the conversion of Hesperidin into high-purity, crystalline Hesperetin is reported.
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