A simple and rapid high performance liquid chromatography (HPTLC) technique has been developed for the determination of flavonoids in Clerodendrum viscosum vent roots. The successive solvent extraction of clerodendrum vicosum vent roots was carried out using petroleum ether, ethyl acetate, chloroform and ethanol by maceration process. The Clerodendrum viscosum root powders were subjected to preliminary phytochemical analysis. Based on phytochemical studies, ethyl acetate extract of Clerodendrum viscosum roots was subjected to column chromatography and eluted successively with chloroform and methanol mixture. The fractions obtained were subjected to HPTLC analysis to identify flavanoids in the collected fractions. HPTLC was performed with silica gel 60F254 plates with solvent system chloroform: methanol (11.8:0.2 v/v) as mobile phase. Detection of flavonoid compound was performed by scanning the developed plate at 366 nm. The results indicated that fractions obtained with mixture of 90% and 80 % chloroform in methanol mixture showed single band of flavonoid compound with Rf value 0.25. Therefore, HPTLC is simple and rapid technique and can be used to determine the bioactive compounds in the plant extracts.