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In vitro antibacterial proprieties of aqueous extract and essential oil of Eucalyptus globulus against multi-resistant Klebseilla pneumoniae isolated from hospitalized patients | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

In vitro antibacterial proprieties of aqueous extract and essential oil of Eucalyptus globulus against multi-resistant Klebseilla pneumoniae isolated from hospitalized patients

Author(s): Marwa Bouras, Naffisa Boutefnouchet Abbaci and Salima Bennadja

The present study was conducted to evaluate the antibacterial activity of aqueous extract and essential oil obtained from Eucalyptus globulus leaves. The aqueous extract was obtained by decoction in volume of distilled and essential oil by hydrodistillation method. The antibacterial effect was studied in vitro in five strains: Klebseilla pneumoniae ATCC10031 and four multi-resistant strains of Klebseilla pneumoniae isolated from hospitalized patients. Minimum inhibitory concentration (CMI) and minimum bactericidal concentration (MBC) was determined by agar dilution method. The essential oil has demonstrated a good antibacterial activity against all strains tested with best inhibition zone equal to 18,5±2,12mm for 5?¥l and 32,50±0,70mm for 10?¥l. Studied aqueous extract showed a considerable antibacterial effect less than essential oil of the some plant, when the best inhibition zone was 10,0±1,41mm for 5?¥l and 13,5±0,70mm for10?¥l. The MIC and MBC of essential oil was ranging respectively from 100 to 400?¥g/ml and 200?¥g/ml to 500?¥g/ml, and for aqueous extract from 300 to 400?¥g/ml and 400 to 500?¥g/ml. The results obtained indicated that Eucalyptus globulus essential oil could be used as a potential source of naturel antibiotic for raising problems of infectious diseases caused by multi-resistant Klebseilla pneumoniae after testing the toxic effects on human.


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