The aims of this research were analysis of the bioactive chemical products and evaluation of antibacterial and antifungal activity. Fifty one bioactive compounds were identified in the methanolic extract of Acinetobacter baumannii. GC-MS analysis of Acinetobacter baumannii revealed the existence of the Malonic acid ,mononitrile , monothioamide, Oxime-,methoxy, 1,2,3-Propatriol, 1-indol-4-yl ; Propenoic acid , 3-[(phenylmethyl)sulfonyl]- , methyl ester, Cis-Pinen-3-ol, 3-Benzoylmethyl-3-hydroxy-5-nitro-2-indolinone, 3-(1-Cyclopentenyl)furan , 3- Benzoylmethyl-3-hydroxy-5-nitro-2-indolinone, 2-Phenylethanamidine, Cyclohexane, 1,3-butadienylidene- , Benzenemethanol, 2-(2-aminopropoxy)-3-methyl, 4H-1-Benzopyran-4-one, 6,7-dimethoxy-3-phenyl-, Cyclopentolate, Levmetamfetamine, Ethanamine,2-(2,6-dimethylphenoxy)-N-methyl, (3-Ethoxy-4,5-dihydroisoxazol- 5-ylmethyl)-amine, DL-Leucine , N-glycyl- , 3,6,9,12-Tetraoxatetradecan-1-ol , 14-[4-(1,1,3-tetramethyl , Phenethylamine , N-hexyl , Dithiocarbamate ,S-methyl-,N-(2-methyl-3-oxobuty , N-Methyl-4-phenyl-butyramide , Hydrazinecarboxamide ,2-[3-cyclohex-3-en- , Hydrazinecarboxamide,2-[3-cyclohex-3-en-, Pterin-6-carboxylic acid , Benzofuran-2-one , 4-amino-2,3-dihydro-, Cyclopentanecarboxylic acid , 1-phenyl -,2-(diethylamino)ethy, Ethanamine , 2-(2,6-dimethylphenoxy)-N-methyl-, Diaziridinone, (1,1-dimethylethyl)(1,1-dimethyl-2-phenylethyl)-, dl-Alanyl-dl-serine, 4-Ethoxy-6-methylhexahydropyrimidin-2-thione, 4H,5H-Pyrano[4,3-d]-1,3-dioxin , tetrahydro- 8a-methyl-, Eicosanoic acid, phenylmethyl ester, Pyrrolo[1,2-a]pyrazine-1,4-dione , hexahydro-, Dibutyl phthalate, Pyrrolo[1,2-a]pyrazine-1,4-dione , hexahydro-3-(2-methylpropy, Pterin-6-carboxylic acid, Cytidine , 5-methyl-, 12- Methyl-oxa-cyclododecan-2-one, 3,7-Diazabicyclo[3,3,1]nonane, 9,9-dimethyl-, Actinomycin C2, 4-Azepan-1-yloxazolidin- 2-one, 4-Carbamoyl-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl, i-Propyl 9-tetradecenoate, 4,4,6- Trimethyl-2-(N-methyl-m-chlorophenylamino)-5,6-dihydro, 2-Butenoic acid , 4-(morpholin-4-yl)-, methyl ester, 2,5- Piperazinedione , 3,6-bis(2-methylpropyl)-, l-f Leucyl -d-leucine, 3H-3a-Azacyclopenta[a]undene -2-carbonitrile ,3- oxo-1-(piper, and Phthalic acid , di(2-propylpentyl) ester. The results of anti-fungal activity produced by Acinetobacter baumannii showed that the volatile compounds were highly effective to suppress the growth of Aspergillus flavus. Acinetobacter baumannii produce many important secondary metabolites with high biological activities.