In previous work, three flavonoids viz Luteolin-7-O - β -glucopyranoside (I), Quercetin-3-O- β -d-glucuronide (II) and Formononetin-7-O- β -D-Glucoside (III) were isolated from Cassia tora l eaves. As an extension of work, the present study aimed to evaluate the anti-oxidant and antica ncer potential of these flavonoids using in-vitro m odels. The ethanol extract was standardized using HPLC by comp aring with known markers. The total phenolic conten t and flavonoid content was accessed using Folin-Denis an d AlCl 3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitric oxide scavenging methods. Breast cancer (MCF7) cell line was used as the in-vitro cancer model for MTT assay . In the HPLC analysis, 4 flavonoids were identified by comp arison with retention time of standard marker viz., quercetin, kaempferol, formononetin and luteolin. The total ph enolic content and flavonoid content was found to b e 18.60 % w/w and 9.5% w/w respectively. The radical scavengi ng activity of the isolated flavonoids decreased in the following order: quercetin (IC 50 values 15, 14, 18 μ g/mL) > luteolin (IC 50 values 19, 21, 14 μ g/mL) > formononetin (IC 50 values 20, 23, 18 μg/mL) respectively. The flavonoi ds luteolin and quercetin possess potent anticancer property against breast cancer cells (MCF7) with IC 50 value of 14 and 19 μ g/mL respectively. This study suggests potential antioxidant and anticancer activities of the flavonoids of Cassia tora.
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