In vitro antioxidant and anticancer activity of flavonoids from Cassia Tora linn. leaves against human breast carcinoma cell lines | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.


In vitro antioxidant and anticancer activity of flavonoids from Cassia Tora linn. leaves against human breast carcinoma cell lines

Author(s): Vijayalakshmi A.* a , Masilamani K. b , Nagarajan E. c and Ravichandiran V. d

In previous work, three flavonoids viz Luteolin-7-O - β -glucopyranoside (I), Quercetin-3-O- β -d-glucuronide (II) and Formononetin-7-O- β -D-Glucoside (III) were isolated from Cassia tora l eaves. As an extension of work, the present study aimed to evaluate the anti-oxidant and antica ncer potential of these flavonoids using in-vitro m odels. The ethanol extract was standardized using HPLC by comp aring with known markers. The total phenolic conten t and flavonoid content was accessed using Folin-Denis an d AlCl 3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitric oxide scavenging methods. Breast cancer (MCF7) cell line was used as the in-vitro cancer model for MTT assay . In the HPLC analysis, 4 flavonoids were identified by comp arison with retention time of standard marker viz., quercetin, kaempferol, formononetin and luteolin. The total ph enolic content and flavonoid content was found to b e 18.60 % w/w and 9.5% w/w respectively. The radical scavengi ng activity of the isolated flavonoids decreased in the following order: quercetin (IC 50 values 15, 14, 18 μ g/mL) > luteolin (IC 50 values 19, 21, 14 μ g/mL) > formononetin (IC 50 values 20, 23, 18 μg/mL) respectively. The flavonoi ds luteolin and quercetin possess potent anticancer property against breast cancer cells (MCF7) with IC 50 value of 14 and 19 μ g/mL respectively. This study suggests potential antioxidant and anticancer activities of the flavonoids of Cassia tora.


Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Recommended Conferences

14th World Congress on Chemistry
Barcelona, Spain

19th European Organic Chemistry Congress
London, UK
Google Scholar citation report
Citations : 11575

Der Pharma Chemica received 11575 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry