Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Inhibition Activity of HMG-CoA Reductase by Rice Brain Extract and Its Fractions as Anticholesterolemia In vitro Study

Author(s): Qomariah Hasanah, Hasim, Didah Nur Faridah, Dimas Andrianto

HMG CoA reductase inhibition effect of rice bran extract and its fractions were examined. Successive extraction was done by using ethanol, followed by successive fractionation using n-hexane, dichloromethane, ethyl acetate, and water for fractions with highest activity. Anti HMG CoA reductase activity of the extract and its fractions were determined spectrophotometrically by NADPH oxidation, using HMG-CoA as substrate. Rice bran extract and its fractions showed more than 50% inhibitory effect on enzyme activity. The extract of rice bran showed 51.44% inhibitory effect on HMG CoA reductase activity. The water fraction from ethanol extract gave the strongest activity (64.54% inhibition). Kinetic study of the enzyme was performed on one concentrations of water fraction (0.05 mg mL-1). These active fractions showed uncompetitive inhibition in Lineweaver-Burk plot analysis. Under standard condition, Km value for non-inhibitor was Km 331.45 mm and Vmax value was 1.3 mm min-1, while with inhibitor Km value was 56.57 nmm and Vmax 0.3 mm min-1.


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