Acne vulgaris is a common skin disorder characterized by non-inflammatory and inflammatory lesions. This study has been taken to investigate in vitro antiacne activity of ethanolic extract of Boswellia serrata gum and its oil. In this study, the selected Boswellia gum extract were evaluated against the Propionibacterium acnes, Staphylococcus epidermidis. These bacteria are the main cause for acne. Agar well diffusion method is used for antiacne study and inhibitory activity is checked against P. acne and S. epidermidis compared to the marketed tetracycline disc and DalacinTM (1% clindamycin phosphate solution) as positive control. From this study it was concluded that B. serrata oil is more effective than B. serrata gum and hence it can be used for topical therapy of acne vulgaris.