The present study was designed to evaluate the in-vitro cytotoxic activity and anthelmintic effect of chloroform extract of Antigonon leptopus Hook & Arn. Cytotoxicity was determined using 3-(4, 5–dimethyl thiazol–2–yl)–2, 5– diphenyl tetrazolium bromide (MTT) assay and IC50 (inhibit cell growth by 50%) was calculated against A-549 (Human lung adenocarcinoma epithelial cell line) and CHOK-1 (Chinese Hamster Ovary cell line) cell lines. Different concentrations (25, 50, 100 mg/ml) of chloroform extract were used for anthelmintic activity against Pheritima posthuma. Effect of inhibition of cell growth showed significantly cytotoxicity against CHOK 1 cell line (Chinese Hamster ovary cell line) with an IC50 (inhibit cell growth by 50%) of >1000 ± 0.00 μg/ml and against and also A-549 cell line (Human Lung adenocarcinoma epithelial cell line) with an IC50 of >1000 ± 0.00. The results obtained from the study indicate significant anthelmintic activity against Pheretima posthuma. In conclusion, the present plant Antigonon leptopus can be considered as an important source of natural products that have potent anthelmintic activity, due to presence of various phytochemical components but it’s not having significant cytotoxic or anticancer activity on cell lines.