Isolation and characterization of DNA fragments locus pyrA (iviI)
Salmonella typhimurium and expression analysis of carbamoyl
phosphate synthetase enzyme

Richardo Ubyaan; Epiphani I. Y. Palit; Jukwati; Lily R. Bekty; Rosye H. R. Tanjung; Yohanis Ngili

Abstract

Isolation and characterization of DNA fragments locus pyrA (iviI) Salmonella typhimurium and expression analysis of carbamoyl phosphate synthetase enzyme

Author(s): Richardo Ubyaan, Epiphani I. Y. Palit, Jukwati, Lily R. Bekty, Rosye H. R. Tanjung and Yohanis Ngili

Typhoid fever is a disease caused by infection with gram-negative bacteria Salmonella typhi. iviI gene is one of the genes was found and has homology with carAB operon of E. coli K12. This gene locus contained in pyrA which serves to express the enzyme carbamoyl phosphate synthetase. The specific objective of this research the isolation and characterization of some genes iviI DNA locus pyrA by amplification of the desired part of the gene using the PCR method. The results of analysis of S. typhi, indicating homologous 829 bp and 59 bp do not homologue, which consists of 37 bp mutated transversions and 22 mutated transition. Analysis on carAB operon of E. coli K12 showed 756 and 132 bp homologous not undergo homologous consisting of: 80 bp mutated transitional and 52 mutated transversional. Analysis of amino acid sequence homology of 0.9 kb fragment (295 amino acids) of the amino acid sequence of S. typhimurium LT2 in GenBank showed that 228 amino acids homologous and 7 amino acids are not homologous. Analysis of the amino acid sequence of S. typhi in Genbank showed homologous 256 amino acids and 39 amino acids are not homologous. While the amino acid sequence of E. coli K12 in GenBank showed homologous 277 amino acids and 20 amino acids are not homologous.

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