Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Abstract

Isolation and Identification of Bioplastic Producing Bacteria from Soil at the Top of Marapi Volcano Mountain, West Sumatra, Indonesia

Author(s): Akmal Djamaan, Anthoni Agustien, Resha Gemeidiya, Miftahul Jannah, Asiska PD and Wangi QA

The purpose of this research is to isolate and identify a bioplastic-producing bacteria namely P(3-hydroxybutyrate) [P(3HB)] from soil at the top of Marapi Volcano Mountain, in West Sumatra, Indonesia. Soil samples was taken by doing purposive sampling technique. Then, it was inoculated in oil palm-bacto-agar medium to obtained bacteria colony that has potential to produces bioplastic P(3HB) by using Nile Blue A as a stain reagent. Results showed there are 40 single colony isolated. Beyond them were 10 isolates that indicated as yellow fluorescent under ultraviolet light as indicated the bacteria accumulated P(3HB) granule in the cells. The 10 isolates then identified by macroscopic, microscopic, biochemistry test. The result were 6 bacteria genera, i.e Bacillus sp 1, Bacillus sp 2, Bacillus sp 3, Bacillus sp 4, Bacillus sp 5 and Bacillus sp 6.


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