Isolation, characterization and biological activities of exopolysaccharide produced by Bacillus marinus

Osama; H. El Sayed; Abd El Kader; M. El-Sayed; Hassan M. Salem; Manal; G.Mahmoud; Mohsen S. Asker; Saher; S. Mohamed

Abstract

Isolation, characterization and biological activities of exopolysaccharide produced by Bacillus marinus

Author(s): Osama, H. El Sayed, Abd El Kader, M. El-Sayed, Hassan M. Salem, Manal, G.Mahmoud, Mohsen S. Asker and Saher, S. Mohamed

The exopolysaccharide from Bacillus marinus is of study interest due to its wide spectrum bioactivity and high production (8.2g/L), so the strain isolated and identified based on morphological and characteristics. The EPS was obtained through the ethanol precipitation from fermentation broth and fractionated by anion-exchange and gelfiltration chromatography. The main fraction of EPS (BMEPS) was determined as homogenous with a molecular weight of 500 kDa. Its structural characteristics were investigated and elucidated by periodate oxidation, Smith degradation, HPLC, FT-IR. It was contained sulfate group (20.2%) and composed of glucose and glucouronic acid in a molar ratio of 3:1, respectively. Small amount of glycerol and large amount of liberated erythritol partially prove the presence of the β-(1−4) linkages between the monosaccharides in the backbone of the polysaccharide. The chemical analysis showed that the proposal structure could be as [Glc-β-(1−4)-Glc-β-(1−4)-Glc.A]n. The biological activities of crude EPS and BMEPS as antiviral against MDCK and H1N1 virus and antioxidant were done. In addition, the antitumor against human breast adenocarcinoma cell line (MCF-7) and human alveolar basal epithelial cell line (A-549) was evaluated by MTT assay. The results suggested that F-6 could be considered as an effective natural antiviral, antioxidant and antitumor sources.

PDF

Select your language of interest to view the total content in your interested language