Methylation technique used for the identification of seed polysaccharide structure from Cassia hirsuta Linn. plant

R. B. Singh

Abstract

Methylation technique used for the identification of seed polysaccharide structure from Cassia hirsuta Linn. plant

Author(s): R. B. Singh

Water soluble seeds polysaccharide was extracted from Cassia hirsuta Linn. plant by acid hydrolysis with sulphuric acid and identification by column and paper chromatographic analysis to be composed of D-galactose and D-mannose in 1:4 molar ratio. Methylation studies of seeds polysaccharide were carried out by Hakomari’s and Purdie’s method and its Infrared spectroscopy indicated that the polysaccharide was a galactomannan. D-mannose residues linked with (1®4)-β type on main polymer chain while D-galactose and D-mannose residues with (1®6)-α type linkages at non-reducing end. Methylated galactomannan on acid hydrolysis with sulphuric acid gave certain medicinal chemicals like methyl sugars as: 2, 3, 4, 6- tetra-Omethyl- D-galactose; 2,3,6-tri-O-methyl-D-mannose and 2,3-di-O-methyl-D-mannose is 1:3:1 molar ratio. On the basis of above finding methylation results a tentative polysaccharide structure has been proposed for the water soluble Cassia hirsuta Linn. seeds.

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