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Micellar Liquid Chromatographic Method Development for Determination of Rosuvastatin Calcium and Ezetimibe in Pharmaceutical Combination Dosage Form | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Abstract

Micellar Liquid Chromatographic Method Development for Determination of Rosuvastatin Calcium and Ezetimibe in Pharmaceutical Combination Dosage Form

Author(s): Smita Sharma, M. C. Sharma, D. V. Kohli, S. C. Chaturvedi

This paper described validated, rapid, simple and sensitive liquid chromatographic procedure that use micellar mobile phase containing only Tween-20 and n-Butanol, is reported for the determination of method for estimation of Rosuvastatin Calcium (ROS) and Ezetimibe (EZE) in tablet dosage form. HPLC separation was achieved on Licrosphere C18 column (250 x 4.6mm) using Tween-20 and n-Butanol Phosphate buffer, pH 5.1 (60:20:20 v/v) at flow rate of 1.0 ml/min at 25oC temperature. Quantitation was achieved by UV detection at 314 nm over the concentration range 5-10 mg/ml for both the drugs with mean recoveries of 99.91% + 0.12 and 100.11% + 0.29 for ROS and EZE respectively. This method is simple, precise and sensitive and it is applicable for the simultaneous estimation of ROS and EZE in tablet dosage form.


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