GET THE APP

Micronucleus assay and pro-oxidant status of patients with known chromosomal aneuploidy | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

Abstract

Micronucleus assay and pro-oxidant status of patients with known chromosomal aneuploidy

Author(s): Eman R. Youness, Hanan F. Aly,El-Bassyouni H. T., Angie M. S. Tosson, Eid M. M., Eid O. M., El-Kotoury A.and Sherien M. El-Daly

Aneuploidy is a well known chromosomal abnormality, and it is considered as a major cause of congenital birth defects and miscarriage. The present study aims to examine micronucleus frequency (MN), oxidative stress and antioxidant activity in chromosomal aneuploidy disorders including Down syndrome (DS), Klinefelter (KF) and Turner syndrome (TS), comparing to healthy controls matched age group. Peripheral blood samples were collected from 50 subjects, including, 20 normal healthy control and 30 patients with Down, Klinefelter and Turner syndromes. Micronucleus level was scored for each subject. Biochemical parameters, including lipid peroxide (LPO), nitric oxide (NO), total antioxidant capacity (TAC) were determined .The activity of paraoxonase1 (PON1) was also detected in blood serum for the three genetic diseases. DS and KF syndrome cases had an elevated count of MN (13.27 ± 0.59, 14.30±1.29 respectively vs. 1.80 ± 0.19; P < 0.05). However, Turner patients recorded an insignificant change in micronucleus frequency. In addition, significant decrease in TAC and PON1 was recorded in all patients comparing with normal controls (p≤0.05). While, NO and MDA product declared significant increase in DS, KF and Turner syndrome as compared to normal control group. Thus, it could be concluded that the MN, antioxidants and oxidative stress biomarkers assays are a powerful tools for detecting genetic disorders in chromosomal aneuploidy patients.


PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 23677

Der Pharma Chemica received 23677 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry