Several new promising bioactive derivatives of 1-(2,5-dihydro-2-methyl-5-phenyl-1-(1,4,5-triphenyl-1H-imidazol-2-yl)-1H pyrrol-3-yl) ethanone were synthesized under microwave irradiations. The compounds were obtained in excellent yields via one pot synthesis method. All the titled compounds were characterized by IR, 1H-NMR, 13C-NMR and mass spectral analysis. Synthesized compounds were evaluated for their preliminary antibacterial and antifungal activity. Antibacterial activity against bacterial strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa using ciprofloxacin as standard drug indicated that compounds 4c, 4h, 4k, 4l exhibited moderate to high potency against the parent compound while more potent than the standard drug. Antifungal studies were carried out against fungal strains like Candida albicans and Aspergillus niger using fluconazole as standard drug indicated that all compounds exhibited high potency against the standard compound except 4a, 4b, 4d, 4g, 4j.