The southern region of the provinces of Papua that includes Merauke and Diegul Boven regency, has the potential of natural resources in the areas of inland waters. One of the many fish species that bring financial benefits are arowana fish. This fish is mostly found in the sout hern region of Papua and the price is quite expensi ve for a particular variant. Traditional fishing patterns an d local knowledge-based society should continue to be pursued in order to improve the productivity of arowana fish. The existence of arowana fish in Papua are common i n the southern region due to the large flow of creeks and major rivers in the region. The existence of arowa na fish are only found in the southern region of Papua affect t he genetic diversity of the fish. The purpose of th is study is to analyze and characterize the pattern of nucleotide mutations biomolecular Arowana fish on the hypervar iable region. Primers designed for amplification of 1 kb arowana fish along the D-loop region of mitochondri al. Based on the results obtained by analysis of mitochondrial D NA nucleotide sequence arowana fish in the D-loop r egion through the direct sequencing of samples arowana fi sh. Number of dominant mutations in Papua arowana f ish as many 9 positions on the D-loop region of mtDNA fish . Nucleotide sequence analysis of the variability o f the sample arowana fish in southern waters of Papua provide id entifying information mutations were highly variabl e in both positions, quantity, and type of mutation that occu rs. The study results that have been obtained are d escribe variants arowana fish that are in the local waters of Papua, in the southern region of Papua; has successfully illustrates the genetic map of the fish through the design of an ef ficient and comprehensive primer on the overall mit ochondrial arowana fish. From total mutations, mutations are u nique ie transversion substitution mutation at posi tion n + 2, changes into a cytosine nucleotides adenine, A → c. Overall output of the data obtained from this s tudy is very useful for the development of biotechnology in part icular fisheries primer design methods and techniqu es of fish reproducible DNA isolation.
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