Multiresidue Analysis of Organochlorine and Organophosphate Pesticides in Bovine Milk  Using Modified QuEChERS Method by GC-MS

Gyansagar Kushwah

Abstract

Multiresidue Analysis of Organochlorine and Organophosphate Pesticides in Bovine Milk Using Modified QuEChERS Method by GC-MS

Author(s): Gyansagar Kushwah

The present study was undertaken for the determination of the residual concentration of OCPs and OPPs in milk by GC-MS. In the present study, residues of OCPs viz. α, β, γ and δ-BHC, heptachlor, aldrin, heptachlor epoxide, endosulfan-I, cis-chlordane, trans-chlordane, p, p-DDE, Dieldrin, Endrin, Endosulfan-II, p, p-DDD, Endrin aldehyde, Endosulfan sulphate, p, p-DDT, Endrin ketone and methoxychlor were evaluated in milk samples. Dichlorvos, Mevinophos, Demeton, Ethoprophos, Naled, Phorate, Diazinone, Disulfoton, methyl parathion, Ronnel, Chlorpyrifos, Fenthion, Trichloronat, Stirifos, Tokuthion, Merphos, Fensulfothion, Bolstar, Azinophos-methyl and Coumaphos residues belonging to the organophosphate group of pesticides were also evaluated in these samples. Sample extraction was performed by adopting the modified QuEChERS principle and cleaned up using a dispersive solid-phase extraction C-18 cartridge. The performance of the GCMS method was investigated for linearity, accuracy, precision, detection limit and quantification limit. Good linearity with correlation coefficients (r2) ranging from 0.996 to 0.999 was obtained for various organochlorine components. The mean recoveries for all OCPs and OPPs components ranged between 72% to 108% and 71% to 101%, respectively. From the total collected 70 milk samples, 5 samples from each target aera were tested for various OCPs and OPPs residues. In this study observed that 11.43% (4/35) of the milk samples tested positive for Endosulfan-I, Endosulfan-II, heptachlor, α-BHC and δ-BHC. The contamination percentages for p, p'-DDD, methoxychlor, endrin, heptachlor epoxide and p, p-DDE were 22.86% (8/35), 17.14% (6/35), 14.29% (5/35), 8.57% (3/35) and 5.71% (2/35) respectively. On the other hand, Mevinphos, Ethoprophos, Azinphos-methyl, Chlorpyrifos and Coumaphos were found to be present in approximately 25.71% (9/35), 28.57% (10/35), 20.00% (7/35), 31.43% (11/35) and 51.43% (18/35) of the milk samples, respectively.

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