Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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New Validated Rp-HPLC Method for the Estimation of Dobutamine In Pharmaceutical Formulation

Author(s): Sivanadh

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Dobutamine in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1.5ml/min was employed on symmetry Shimadzu LC-20 ATVP Kromasil C-18 column at ambient temperature. The mobile phase consisted of Methanol: 0.01MPotassium di hydrogen Phosphate(60:40 v/v). The UV detection wavelength was 280nm and 20 μl sample was injected. The run time for Dobutamine is 6 min. The flow rate was found to be 1ml/min. Calibration graph was found to be linear at range 0.2mg/ml to 1.0mg/ml. The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the routine quality control analysis of Dobutamine formulations, which could be the better choices compared to the reported methods of literature


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