For the estimation of Rabeprazole in bulk and tablet dosage form two simple and sensitive methods have been developed. In Method 1 folin–ciocalteu reagent and sodium hydroxide is used. Folin–Ciocalteu reagent reduced in the presence of sodium hydroxide by the Rabeprazole and blue coloured chromogen is formed, exhibiting absorption maxima at 640 nm and at a concentration range of 1–8 μg/ml linearity was obtained with correlation coefficient (r2) 0.9983. In method 2 rabeprazole reduced ferric nitrate. The reduced ferric ions reacts with 2,2, bipyridine, exhibiting absorption maximum at 522 nm and at concentration range of 2-16 μg/ml linearity was obtained with correlation coefficient (r2) 0.9994. According to ICH guidelines these two methods are validated and the methods were suitable for estimation of Rabeprazole in bulk and pharmaceutical dosage form.
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