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Novel Electrochemical behavior of 3-(5-(3-Methyl-1-phenylpyrazolazo)-1- nitroso-2-naphthol and use it for Spectrophotometric Determination of Iron(III) in Blood samples | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Novel Electrochemical behavior of 3-(5-(3-Methyl-1-phenylpyrazolazo)-1- nitroso-2-naphthol and use it for Spectrophotometric Determination of Iron(III) in Blood samples

Author(s): Fatma A. Khazaal and Hussein J. Mohammed

The azo reagent3-(5-(3-methyl-1-phenyl pyrazolazo)-1-nitroso-2-naphthol was synthesized and characterized by FT-IR ,1H-NMR and 13CNMR spectral techniques. The electrochemical behavior of the azo reagent and its complex with Fe(III) has been studied at glassy carbon disk GCE electrode in different supporting electrolyte at concentration (1M) and scan rate (100mvs-1). Spectrophotometric determination of Iron (III ) is based on the formation of a 2:1 complex with above reagent. The complex has λmax at(454) nm and εmax of( 1.7982*104)L.mol- 1.cm-1 . A linear correlation (0.1 – 3.0 μg. ml-1) was found between absorbance at λmax and concentration . The effect of diverse ions on the determination of copper (II) to investigate the selectivity of the method were also studied . The stability constant of the product was (2.869*108 ) . The proposed method was successfully applied to the analysis of honey sample .


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