The present work is devoted to study the effect of various factors affecting the enzymatic synthesis of fatty acid xylose ester using the methodology of experimental designs. To optimize the different parameters that have a direct influence on the production of lauric xylose ester, experiments were conducted using first a design of Plackett- Burman to initially screen five of which the three factors were found to have significant effect on xylose ester production. Response surface methodology under Box-Behnken design was then employed to analyze the effects of important parameters on the preparation of fatty acid xylose ester. Experimental ester conversions were found to be in good agreement with predictions. The search for optimal conditions, verified by analysis of contour plots, helped to locate the optimal value of the conversion, C = 85 %. This corresponds to the following values of the factors : temperature 60°C, time 72 hours, and 30 mg of molecular sieves.
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