Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Phytochemical extraction, optimization and physico-chemical characterization of two bioactive isolates from the leaves and stem of Cissampelos pareira

Author(s): K. Nagarajan, Nithish Chauhan, Ashu Mittal, Vijendar Singh, Ramesh B. Bodla and Raj Kumar Tiwari

In the traditional Indian medicine, the roots and leaves of Cissampelos pareira are administered for the relief of diarrhoea and antiseptic against inflammation. The present investigation is to optimize the suitable extraction method and thereby isolating and characterizing various bioactive leads from the leaf and stem portion. Preliminary phytochemical screening reveals the presence of maximum alkaloids and moderate quantity of flavonoids and saponins with dichloromethane as solvent. In leaf portion, the hot extraction method was suited as the alkaloid content seems to be maximum, whereas in stem portion, the cold maceration was suited with more flavonoid content. This was evidenced further with higher yield values obtained for leaf extract (56% w/w) rather than stem extract (39.5% w/w). Two pure bioactive leads were isolated, each one from leaf (CP*) and stem (CP**). CP* of leaf (80% CHCl3-MeOH) was identified as alkaloid by qualitative analysis (Dragendroff's reagent); TLC analysis (Rf: 0.88, Toluene: EtoAc: GAA (7:2:1)); U.V analysis (lmax: 265nm, Chromophore: C=N; Transition: p-p* (forbidden)); I.R spectra (2919-2850 cm–1; 2346 cm–1; 1724 cm–1; 1166 cm–1; 720 cm–1) and Mass spectra (M-229) fragmentation peak for the presence of Bebecrine after elucidation. In addition CP** of stem (60% CHCl3–MeOH) was identified as isoquinoline alkaloid by qualitative analysis (Hager's reagent); TLC analysis (Rf : 0.95; Toluene: EtoAC: Diethylanine (7:2:1); U.V analysis (lmax: 265nm); I.R. spectra (3774–3378 cm–1; 2926–2974 cm–1; 23.47–2132 cm–1; 1927 cm–1; 1728–1665 cm–1; 1378–1256 cm–1; 1089–1049 cm–1; 880–758 cm–1); and Mass spectra (M+1 607) for the presence of cissampareine, which is reported for the first time from stem portion.


PDF

FIND ARTICLES
SCImago Journal & Country Rank
 
Erkek öyle sert ve acımaz sikiyor ki bayan çaresiz bir şekilde tırtıl misali sikiş yataktan aşağı doğru kayıyor fakat hem adam hemde kadın aşırı mobil porno derecede zevk alıyorlar Uzun zamandır karısı ile ilgili planları olan türk porno adam onu başkaları sikerken hayal ediyor bu durumu karısından sikiş izle gizliyordu fakat bir gün adamın moralinin bozuk olduğunu anlayan karısı sikiş ona ne olduğunu sordu ve adamda ona her şeyi anlatmıştı karısı bu porno resim durumdan Büyük zenci yarağı sevenler için özel olarak eklenmiş videomuz türk porno kalın ve damarlı zenci siki arayıp bulamayanlar için özel olarak sikiş izle yayınlanmıştır filmde ise sarışın zengin sürtük büyük mobil sikiş kalın damarlı alet sevdiğini bir çok kez dile getirmiş