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Phytochemical Screening and Evaluation of Antibacterial Activity of Extracts from Leaves and Roots of Echium Vulgare of the Region of Tlemcen | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Phytochemical Screening and Evaluation of Antibacterial Activity of Extracts from Leaves and Roots of Echium Vulgare of the Region of Tlemcen

Author(s): Nesrine Bentabet*, Kebir boucherit, Boucherit-Otmani zahia and Nadjib Rahmoun

Objectives: Our work is part of the search for new natural antibacterial agents from a plant that grows wild in the region of Tlemcen (Remchi) called Echium vulgare and belonging to the family Boraginaceae.

Methods: Triphytochimical study was conducted using several experimental protocols. The antibacterial activity of the different samples was evaluated by two methods: Disk diffusion method and the dilutions against four bacterial strains Gram positive and four Gram negative. The minimum inhibitory concentrations (MICs) were determined by growth in liquid medium microplate (NCCLS).

Results: Phytochemical tests performed in this study revealed the presence of different families of existing chemicals with varied intensity between the two parts of the plant. Yields are higher in leaves compared to roots. The results show that all extracts are endowed with antibacterial activity with inhibition diameters between 07 mm and 22 mm. The calculation of CMI extracts of Echium vulgare against bacteria showed good antibacterial activity (0.95 mg/mL ≤ MIC ≤ 13.5 mg/mL).

Conclusion: The extracts showed variable antimicrobial activities against different bacterial strains tested. This variation can be explained by the variation of the chemical composition of the plant studied.


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