Abstract

Phytochemical Study and Evaluation of the Antioxidant and Antimicrobial Activities of Extracts of the Red Vine

Author(s): Agouazi Ounissa, Bouchenak Ouahiba, Yahiaoui Karima, Ziriri Djamel, Khan Feriel, Arab Karim

The phenolic compound presents in a wide variety of fruits and vegetables have antioxidant and anti-radical activities. The European Medicines Agency recognizes that extracts of leaves, seeds or fruits from red vines are endowed with anti-inflammatory, anti-edematous, diuretic and liver protective properties. The purpose of this study is to demonstrate experimentally the antioxidant and antimicrobial effect of leaves and fruits extracts of a local Algerian vine Ahmar Bouemar, of the region of Benchecao (Medea), to value even more its use traditional. The extraction of polyphenols is made from leaves by maceration in a mixture methanol/water (80:20 v/v). For the fruit, we accomplished test on the juice. Extracts were characterized by High Performance Liquid Chromatography (HPLC). The antioxidant capacity of extract was represented using three methods: the test 2,2-Diphenylpicrylhydrzyl (DPPH). Β-carotene and the frappe (Iron reduction). The antimicrobial effect is tested on six microbial strains responsible for some infectious diseases by diffusion method on the agar medium. The yield of polyphenols got is in the order of 33.13%. Majority compounds are coumarins (24.312%), gallic acid (13.31%) and syringic acid (10.19%). The evaluation of antimicrobial activity of these extracts was very significant. The interpretation of results got by this job brings to light that the studied grape variety has remarkable biological properties besides its organoleptic sought-after qualities who serve in search of the best typicalness of grape varieties minor and who can possibly be promoted to the detriment of recent introduction foreign grape varieties.

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