An electrochemical sensor for the detection of insulin was fabricated, using the electrochemically deposited CCLP- Au NPs modified electrode. The morphology and structure of the CCLP- Au NPs are characterized by Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), UV-Visible spectroscopy (UV-Vis) and Transmission Electron Microscopy (TEM) studies. The building of the immunosensor was evaluated in each and every step by Cyclic Voltammetry (CV) and Impedance spectroscopy (EIS). The electrochemical detection was based on the anti-insulin-HRP (Horseradish Peroxidase) bundled with Au NPs which binds to the immune complex and the response was observed using Hydro Quinone (HQ) and Hydrogen peroxide (H2O2) in phosphate buffer electrolyte. From the results, the sensitivity range is from 50 to 556 pM/l and LOD is calculated as 2.14 pM/l (3σ/s). The developed immunoassay has the ability to insulin in human blood serum samples of healthy individuals.