Production of inulinase by Pichia caribbica using artichoke extract and pure chicory Inulin | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.


Production of inulinase by Pichia caribbica using artichoke extract and pure chicory Inulin

Author(s): Mounira Kara Ali, Serge Hiligsmann and Noreddine Kacem Chaouche

Inulinases constitute an important class of enzymes for producing fructose and fructooligosaccharides, which are extensively used in pharmaceutical and food industry. In this study, two carbon sources were evaluated for their production of inulinase by yeast, Pichia caribbica (KC977491), i.e., pure chicory inulin and artichoke extract. The highest inulinase activity (70 IU/ml) and the good growth cell (DO 38.5 at 600nm) were observed in artichoke extract as a sole carbon and nitrogen source at 37°C within 48 h. Furthermore, the optimal conditions such as pH and temperature, to obtain the maximum stability and activity of the enzyme were identified. The enzyme activity was found to be the highest at pH 3,4 and at 65°C. Results suggested that the artichoke extract induced exoinulinase synthesis in P. caribbica (KC977491) and can be utilized as a potential substrate for inulinase production. Thus, P. caribbica (KC977491) may play an important role in biotechnological domain.


Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry