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Quantitation of urea in urine by Fourier transforms infrared spectroscopy | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
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Abstract

Quantitation of urea in urine by Fourier transforms infrared spectroscopy

Author(s): Syed Ismail Ahmad*, Iizhar Ahmed Syed, P. Ravi Prasad and Adeel Ahmad

The objective of the present works is to quantitate the amount of Urea in Urine by using FT-IR Spectroscopy. The normal urine collected from the healthy male volunteers, different concentrations in the range of 1.25, 2.5, 5 and 10μg/mL were prepared by adding Urea. Then FT-IR spectra were recorded at the region 1500 -700cm-1 . The overall spectra of urine samples are dominated by urea. The spectra reveals that the primary peak at 3400cm-1 and a secondary peak at 1641cm-1 it is due to presence of urea. A graph between concentration of urea and intensity of absorption shows a linear relationship. There is an increase in the intensity of absorption at wave number 1641cm-1. This further confirms the specific peak for Urea


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