Abstract

Quantitative Determination of Esculetin by HPTLC in the Ethanolic Leaf Extract of C. Intybus Linn

Author(s): Rajkumari*, Meenakshi Sharma and Vidhu Aeri

High-performance thin-layer chromatography addresses a more proficient type of ordinary TLC wherein applying, examining, and representation the spots of samples are completely automatic. Taking everything into account, HPTLC is one of the least complex, minimal expense, flexible, and explicit strategies that can be applied for the verification of food supplements including medicinal plant materials. Esculetin, chemically known as 6,7-dihydroxy coumarin, is one of the ingredients of the traditional medicinal plant Cichoriumintybus Linn. In the current investigation, a simple HPTLC method was developed and validated for the separation and quantification of esculetin in the ethanolic leaf extracts of CichoriumintybusLinn., via HPTLC. Precoated silica gel 60 F254 was used as stationary phase and toluene: ethyl acetate: formic acid in the ratio of 9.5:8.5:2 v/v as mobile phase. The plate was scanned and quantified at 356 nm for esculetin and the method was validated in terms of linearity, accuracy, and specificity. The proposed HPTLC method gives a quicker and cost-effective control for regular analysis of esculetin in the ethanolic extracts of the plant containing coumarins.

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