Quantization of ascorbic acid in ayurvedic amla capsule by various analytical techniques | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
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Quantization of ascorbic acid in ayurvedic amla capsule by various analytical techniques

Author(s): Vallapudas Hima, S. Rubesh Kumar, N. Duganath and N. Devanna

Phyllanthus Emblica, commonly known as amla used in the ayurvedic preparations of Indian system of medicine. As there are no reported methods for this ayurvedic capsule dosage form of amla, so in this present study, an attempt has been made to develop an analytical method in spectroscopy and TLC for the estimation of ascorbic acid in this formulation. Spectrophotometric estimation of ascorbic acid shows the maximum absorbance at 264nm. Beer's law is obeyed in the concentration range of 1-6 μg/ml. Assay method was developed for the ayurvedic formulation, the percentage purity was found to be 97.68 % w/v. The method was validated as per ICH guidelines and results were statistically calculated. Recovery studies were carried out and the percentage recovery was found to be 99.01 - 100.70%w/v, the system was found to be linear and the correlation coefficient (r2) was found to be 0.9994, method was found to be precise and the relative standard deviation for intraday and interday was found to be 0.122 and 0.163 respectively, detection and quantification limit was found to be 0.3μg/ml and 1μg/ml respectively. TLC method were carried out for identification of ascorbic acid in standard and ayurvedic formulation by using a solvent system of dichloromethane: acetone: GAA (4:3:3 v/v/v) and the Rf value of ascorbic acid was found to be 0.77. The stress degradation studies were performed for both API and Ayurvedic formulation as per ICH guidelines, the degradation was observed in oxidative, photolytic, hydrolytic degradation under acidic, alkaline conditions and dry heat induced studies.


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