The sorption characteristics of bio-adsorbents derived from leaves of Justicia adhatoda , Cissus quadrangularis, Soapnut Acacia toward Chromium (VI) ions , have been explored by varying the physicochemical parameters such as pH, sorbent concentration and time of equilibration. Percentage of extraction of Chromium (VI) is found to be pH sensitive and also depends on time of equilibration and sorbent concentration. Conditions have been optimized for the maximum extraction of Chromium (VI); it is found to be: 88.0% with Justicia adhatoda leaves powders; 88.0% with Cissus quadrangularis leaves powder, and 84.0% with Soapnut Acacia leaves powders. Tenfold excess of common cation ions are found to have synergistic effect in enhancing the % removal of Chromium(VI). SO4 2-, PO4 3- and CO3 2- are interfering in the order: PO4 3- > SO4 2-> CO3 2-. NO3 -, Cl – and F- have marginal interference. The methodologies developed in this work are remarkably successful in removing the Chromium(VI) from industrial effluents and polluted lake samples.