A simple, precise and reproducible reverse phase high performance liquid chromatographic method for the estimation of valproic acid in bulk and soft gelatin capsules was developed and validated. The quantification was carried out using a Nova-pack phenyl, 4μm, 150 mm × 3.9 mm i.d. column, with an isocratic elution at a flow rate of 1.2 ml/min and UV detection at 210 nm. The mobile phase composition was acetonitrile: buffer (30:70, v/v) (pH 2.5). The method was validated for specificity, linearity, accuracy, precision, limit of detection, limit of quantification, robustness and solution stability. The linearity of the proposed method was investigated in the range of 10-1500 μg/ml. Mean inter- and intra-assay relative standard deviations (RSD) were less than 2.0%. The proposed method was successfully applied for the analysis of valproic acid in bulk and pharmaceutical dosage forms.
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