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RP-HPLC method development and validation for simultaneous estimation of bromhexine and ciprofloxacin in tablet dosage form | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
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Abstract

RP-HPLC method development and validation for simultaneous estimation of bromhexine and ciprofloxacin in tablet dosage form

Author(s): Sanjay S. Pekamwar, Tukaram M. Kalyankar, Seema. S. Kokate

Bromhexineis a mucolytic agent, secretolytic while Ciprofloxacin is abroad spectrum antibacterial agent, belonging to the group of fluoroquinolones, both the drugs available in tablet dosage form as BRO 8 mg and CIP 500 mg. The chromatographic separation was achieved on reversed-phase C18 column (5μ, 250 x 4.6 mm, i.d.) in the isocratic mode using methanol: 0.05 M phosphate buffer +0.5 mL tri-ethylamine + 0.5 mL tetra hydrofuran (80:20, v/v), at pH 3.8 adjusted with orthophosphoric acid as the mobile phase at a flow rate 0.8 ml/min. Retention times of BRO and SIP were 4.78 and 3.01 min respectively. Quantitation was achieved with PDA detection at 217 nm. The linearity of BRO and CIP was in the range of 5-30 μg/mL and 50-500 μg/mL respectively. A simple high performance liquid chromatography method is developed to the simultaneous determination of Bromhexine and Ciprofloxacin. Developed method is economical in terms of the time taken and amount of solvent consumed for each analysis. The method is validated and successfully applied to the simultaneous determination of Bromhexine and Ciprofloxacin in bulk and pharmaceutical formulations.


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