GET THE APP

RP-HPLC method development and validation for simultaneous estimation of bromhexine and ciprofloxacin in tablet dosage form | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

Abstract

RP-HPLC method development and validation for simultaneous estimation of bromhexine and ciprofloxacin in tablet dosage form

Author(s): Sanjay S. Pekamwar, Tukaram M. Kalyankar, Seema. S. Kokate

Bromhexineis a mucolytic agent, secretolytic while Ciprofloxacin is abroad spectrum antibacterial agent, belonging to the group of fluoroquinolones, both the drugs available in tablet dosage form as BRO 8 mg and CIP 500 mg. The chromatographic separation was achieved on reversed-phase C18 column (5μ, 250 x 4.6 mm, i.d.) in the isocratic mode using methanol: 0.05 M phosphate buffer +0.5 mL tri-ethylamine + 0.5 mL tetra hydrofuran (80:20, v/v), at pH 3.8 adjusted with orthophosphoric acid as the mobile phase at a flow rate 0.8 ml/min. Retention times of BRO and SIP were 4.78 and 3.01 min respectively. Quantitation was achieved with PDA detection at 217 nm. The linearity of BRO and CIP was in the range of 5-30 μg/mL and 50-500 μg/mL respectively. A simple high performance liquid chromatography method is developed to the simultaneous determination of Bromhexine and Ciprofloxacin. Developed method is economical in terms of the time taken and amount of solvent consumed for each analysis. The method is validated and successfully applied to the simultaneous determination of Bromhexine and Ciprofloxacin in bulk and pharmaceutical formulations.


PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry