Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Screening for active agent to anti-diarrhea by an evaluation of antimicrobial activities from three fractions of sappan wood (Caesalpinia sappan. L)

Author(s): Oktaf Rina, Sanusi Ibrahim, Abdi Dharma, Afrizal and Chandra Utami Wirawati

The object of this research was to evaluate and compare the antimicrobial activities of three fractions sappan wood (Caesalpinia sappan. L.) also known as sappan wood, that was methanol, ethyl acetate, and hexane fractions. The sappan wood was collected from traditional market, minced, extracted by methanol and fractionationtreatment by nhexane continues by ethyl acetate. These fractions were evaluated for antimicrobial activity against bacteria Escherichia coli and Staphylococcus aureus using the cup-plate agar diffusion method in Nutrient Agar medium to determine the minimum inhibitory concentration (MIC). The phytochemical of it extract was also analysis by test identification tests. The phytochemical of sappan wood proved the presence of phenolic, flavonoid, tannins and a little bit of terpenoid and saponin but no alkaloid and steroid compound. Out of the three fractions of sappan wood, the methanol fraction showed maximum average clear zone inhibition against. Antimicrobial activity from the three fraction(n-hexane, ethyl acetate, and methanol) of it wood showed that methanol fraction has the best value. The inhibition maximum concentration value of methanol extract of it to E.coli and S.aureus were 13,7± 0,6 mm and 16,3±2,1 mm, it recommended for use as agent antimicrobial to disease diarrhea earlier.


PDF

FIND ARTICLES
SCImago Journal & Country Rank
 
Erkek öyle sert ve acımaz sikiyor ki bayan çaresiz bir şekilde tırtıl misali sikiş yataktan aşağı doğru kayıyor fakat hem adam hemde kadın aşırı mobil porno derecede zevk alıyorlar Uzun zamandır karısı ile ilgili planları olan türk porno adam onu başkaları sikerken hayal ediyor bu durumu karısından sikiş izle gizliyordu fakat bir gün adamın moralinin bozuk olduğunu anlayan karısı sikiş ona ne olduğunu sordu ve adamda ona her şeyi anlatmıştı karısı bu porno resim durumdan Büyük zenci yarağı sevenler için özel olarak eklenmiş videomuz türk porno kalın ve damarlı zenci siki arayıp bulamayanlar için özel olarak sikiş izle yayınlanmıştır filmde ise sarışın zengin sürtük büyük mobil sikiş kalın damarlı alet sevdiğini bir çok kez dile getirmiş