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Seeds polysaccharide structure from Wrightia tinctoria R.Br. (Roxb.) plant | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
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Abstract

Seeds polysaccharide structure from Wrightia tinctoria R.Br. (Roxb.) plant

Author(s): R. B. Singh

Polysaccharide was extracted from Wrightia tinctoria R.Br. (Roxb.) seeds with water and precipitated with ethanol then hydrolysed by sulphuric acid. After analysis with Gas Liquid Chromatography, Column and Paper chromatographic analysis of hydrolysate was to be composed of D-galactose and D-mannose in the molar ratio of 1:3. Studies of methylation of polysaccharide by Haworth’s, Hakomari’s and Purdie’s method and its IRspectroscopy and 1H and 13NMR Spectroscopy indicated that the polysaccharide was a galactomannan with a chain of D-mannopyranose and D-galactopyranose residue linked β-type with (1→4), which carried alternatively α-type with (1→6)-D-galactopyranose residue. Methylated galactomannan on acid hydrolysis (H2SO4) gave important medicinal chemicals like methyl sugars as: 2,3,4,6-tetra-O-methyl-D-galactose; 2,3,6-tri-O-methyl-D-galactose; 2,3,6-tri-O-methyl-D-mannose and 2,3-di-O-methyl-D-mannose in 1:1:5:1 molar ratio. On the basis of above finding methylation results a tentative polysaccharide structure has been proposed for Wrightia tinctoria R.Br. (Roxb.) seeds.


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