The aim of this study was to examine the antimicrobial susceptibility of Streptococcus pneumoniae isolates from invasive meningitis cases, to determine the serotypes and susceptibility of serogroups/serotypes. Pneumococcal meningitis isolates were isolated from Cerebrospinal Fluid (CSF), between 2012 and 2013. Serotypes were determined using the capsular reaction test (Quellung test). Antimicrobial Susceptibility was tested using the disk diffusion method as determined by the CA-SFM guideline. The evaluation of susceptibility to -lactamins showed that 56% of the strains were penicillin non-susceptible strains, 31% were resistant to macrolides. The isolates were resistant to other antibiotics, 37% to trimethoprim-sulfamethoxazole, 12% to chloramphenicol, 6% was observed with glycopeptides and aminosides. Almost all strains serotyped have a power of resistance with different families of antibiotics tested. Serotypes 2, 5, 6, 16, 12, 19A, 23 had a resistance to β-lactams and macrolides. All of isolates serotypes 6 and 32 were resistant to glycopeptides and aminoglycosides. This study aims to report the resistance evolution of S. pneumoniae strains and serogroups/serotypes with penicillin and different family’s antibiotics from Maroccan pneumococcal isolates.