Selenium is of fundamental importance to human heal th. Differential pulse cathodic stripping voltammet ry was used to determine the selenium concentrations in (140) s amples of human serum from a control group of inhab itants in Tlemcen city (Algeria), the detection limit was 29 μ g/L. The concentration of selenium in human organi sm varies widely between geographical areas depending on its content in soil and plants, dietary Selenium intake , bioavailability and retention, mineral interactions and other factors. The mean selenium concentration in serum was 68.57 μ g/L. Serum selenium concentration in healthy Tlemcenian adult that found in this study is very close to serum concentration of other countries. The results from the analysis of two Seronorm standard referen ce materials showed good agreement with the certified values.