Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry


Simultaneous Determination of Chloroacetanilide Herbicide Residues in Crops with Gas Chromatography-Mass Spectrometry

Author(s): J. Qiu, L.S. Zhang, W.C. Song, S.M. Yang, S.H. Dai, X.H. Xue

A rapid and sensitive method was developed for the simultaneous determination of eight chloroacetanilide herbicides in crops (soybean, rice, and wheat). Herbicides including alachlor, acetochlor, pretilachlor, butachlor, metolachlor, napropamide, diflufenican, and propanil were extracted from samples using a modified QuEChERS method. Acetonitrile containing 0.5% acetic acid was used as extraction solvent, and the extracts were analyzed by gas chromatography-mass spectrometry after being subjected to a simple clean-up procedure using C18 sorbent instead of the primary–secondary amine (PSA). The average recoveries for all eight herbicides were higher than 80% with relative standard deviations lower than 9% in the spiked concentration range of 16~800 μg kg-1 and limits of detection (LODs) ranged from 1 to 4 μg kg-1. The method was applied to analyze some samples obtained from various sources (fields, markets, and families) and a lower residue level of chloroacetanilide herbicides in crops was found. The results showed that the new method is efficient, sensitive, and effective for monitoring residual chloroacetanilide herbicides in crops.