Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry


Simultaneous estimation of Atorvastatin calcium, Ramipril and Aspirin in capsule dosage form using HPTLC

Author(s): Anilkumar Sharma, Binny Shah and Bhavesh Patel

A simple, sensitive, precise and accurate high performance thin layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of atorvastatin (AT) calcium, ramipril (RA) and aspirin (AS) in combined dosage form. The method was developed using precoated silica gel 60F254 as stationary phase. The mobile phase used was a mixture of benzene: ethyl acetate: toluene: methanol: glacial acetic acid (4.0:4.5:1.0:0.5:0.1 v/v/v/v/v). Detection was carried out with ultra-violet detection at 220 nm. The Rf values were about 0.45±0.02, 0.28±0.01 and 0.72±0.02 for AT, RA and AS, respectively. The developed method was validated for linearity, accuracy, precision, limit of Detection (LOD), limit of quantification (LOQ) and robustness. The linearity ranges were 0.5-2.5 μg/spot for AT, 0.5-2.5 μg/spot for RA and 0.75-3.75 μg/spot for AS with mean recoveries of 100.29±0.94, 99.06±1.79 and 98.85±0.61 for AT, RA and AS, respectively. The proposed method can be used for the estimation of these drugs in combined dosage forms.