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Simultaneous Estimation of Formoterol Fumarate Dihydrate and Fluticasone Propionate in Dry Powder Inhalation Formulation by HPTLC | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Simultaneous Estimation of Formoterol Fumarate Dihydrate and Fluticasone Propionate in Dry Powder Inhalation Formulation by HPTLC

Author(s): Narendra M. Gowekar and Shailesh J. Wadher

High performance thin layer chromatographic (HPTLC) method has been developed and validated for simultaneous analysis of Formoterol fumarate dihydrate and Fluticasone propionate in dry powder inhalation formulation. Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60F254, with toluene: ethyl acetate: formic acid (7: 3: 0.1 % v/v/v) as mobile phase. Detection was performed densitometrically at 215 nm. The RF of Formoterolfumarate dihydrate and Fluticasone propionate were 0.19 ± 0.10and 0.41± 0.10, respectively. Linearity was found to be in the concentration range of 50-350ng/spot for Formoterol fumarate dehydrate and 50- 350ng/spot for Fluticasone propionate, accuracy (99.28 % for Formoterol fumarate dehydrate and 99.46 % for Fluticasone propionate) and specificity, in accordance with ICH guidelines. The methods can be used for routine simultaneous analysis of Formoterol fumarate dihydrate and Fluticasone propionate in dry powder inhalation formulation


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