Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry


Simultaneous estimation of lornoxicam and paracetamolin tablets by reverse phase HPLC method

Author(s): Surekha J. Babar, Varsha B. Mane and Satish B. Bhise

A simple,fast, precise and accurate liquid chromatographic method was developed for simultaneous estimation of Lornoxicam and Paracetamol in combined tablet dosage form by Reverse phase HPLC method. Chromatography was carried out on a C-18 column (250 mm×4.6 mm,i.d. 5 μm) using Acetonitrile:0.05 M Ammonium Acetate buffer (pH adjusted to 6.5 with triethylamine) in the ratio of 98:2 (v/v) as amobile phase at a flow rate of 0.8 ml/min and eluents were monitored at 280 nm. The calibration curves were linear over the range of 2 – 20μg/ml for Lornoxicam and 10 – 20 μg/ml for Paracetamol. The average retention time ofLornoxicam and Paracetamol was found to be 2.086 min and 3.70 min respectively. The method was reproducible, with good resolution and sharp peak of Lornoxicam and Paracetamol. Theresults of the analysis have been validated statistically.