Two simple spectrophotometric methods have been developed for simultaneous estimation of Nateglinide (Nat) and Metformin Hydrochloride (Met) from tablet dosage form. First method involves solving simultaneous equations based on measurement of absorbance at two wavelengths 216 nm and 233 nm (λ max of Met). The second method is first order derivative spectroscopic method, wavelengths selected for quantitation were 216.0 nm for Nateglinide and 243 nm for Metformin hydrochloride (zero cross for nateglinide). Beer’s law was obeyed in the concentration range of 0.5-80 mg/ml for Nat and 0.5-40 mg/ml for Met in both the simultaneous equation and first order derivative methods respectively. The results of analysis have been validated statistically and by recovery studies. The utility of the developed methods has been demonstrated by analysis of commercial formulation containing these drugs.
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