Tuberculosis (TB) is the world’s second most common cause ofdeath from infectious disease, next to human immunodeficiency virus (HIV). TB has become an increasing health problem since emergence of HIV; TB/HIV coinfection adds further complications in treating the disease. It is also a major concern for industrialized nations because of emergence of drug resistance, alcohol / drug abuse, growth of immigrants and other factors.Method employs formation andsolving of simultaneous equation using 263 nm and 292 nm as two analytical wavelengths for both drugs inDistilled water. Isoniazidand Pyridoxine at their respective λmax 263 nm and 292 nm showslinearity in a concentration range of 1-11μg /mL and 5-30μg /mL. Recovery studies for Isoniazid 99.56 % and100.14% for Pyridoxine in case of simultaneous equation method confirming the accuracy of the proposed method. Theproposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific.
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