Spectrophotometric determination of erythromycin using charge transfer complexation | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.


Spectrophotometric determination of erythromycin using charge transfer complexation

Author(s): Ukoha O. Pius and Nwanisobi C. Gloria

A simple and sensitive spectrophotometric method is described for the quantitative determination of erythromycin. The method is based on charge transfer complexation reaction of, erythromycin as n-electron donor with 2, 3 dichloro-5,6-dicyano-1,4- benzoquinone (DDQ) as -acceptor to give highly coloured complex with 1:1 stoichiometric ratio. The coloured products were quantified at 464nm under the optimized experimental conditions. Beer’s law is obeyed over the concentration ranges of 5-150μg/ml. The apparent molar absorptivity was calculated to be 1.27 x 103Lmol-1cm-1 and corresponding Sandell sensitivity of 1.56. The limit of detection and quantification were 2.1and 6.4 respectively. The proposed methods were applied successfully to the determination of erythromycin in pure and commercial forms with good average recovery of 105.4 %. Statistical comparison of the result was performed with regards to accuracy and precision using student’s t-test and f-test at 95% confidence level.


Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry