Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Spectrophotometric determination of Nateglinide using 2,4-Dinitrophenyl Hydrazine and Potassium Ferricyanide in pharmaceutical dosage form

Author(s): M. Sireesha, R.S. Chandan*, B.M. Gurupadayya and A. Shravya

Two simple, extraction free spectrophotometric methods (Method I and II) for the quantitative estimation of nateglinide in bulk drugs and pharmaceutical formulations (tablets) have been developed. The first method is based on the oxidation of 2,4- dinitrophenyl hydrazine (2,4-DNP) and coupling of the oxidized product with drugs to give intensely colored chromogen. Condensed product of Nateglinide at its λmax 480 nm shows linearity in the concentration range of 30-100 μg/ml. The second method is based on the formation of green color complex between the drug (Nateglinide) and 0.5% w/v ferric chloride and 0.1% potassium ferricyanide. The green colored complex showed maximum absorbance at 725 nm and linearity was observed in the concentration range of 5-30 μg/ml respectively. The relative standard deviation for first method is 0.30% and for second method is 0.32% was obtained. Linear relationships with good correlation coefficients (0.999) were found between absorbances and the corresponding concentrations of the drug. The percentage recovery was ranged from 99.2 and 98.6 for method- I and II respectively. The results of analysis for the two methods have been validated statistically and by recovery studies.


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