Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Abstract

Spectrophotometric Determination of Tinidazole Using Promethazine and Ethyl Vanillin Reagents in Pharmaceutical Preparations

Author(s): Nabil .A .F. Alhemiary and Mohammed. H. A. Saleh.

Two simple sensitive and reproducible spectrophotometric methods have been developed for the determination of tinidazole either in pure form or in their tablets. The proposed methods are based on the reduction of the nitro group to amino group of the drug. The reduction of tinidazole was carried out with zinc powder and zinc dust and concentrated HCl at 90 ± 5 °C for 15 min in water. Method A is based on Schiff's basses reaction used ethyl vanillin reagent and measurement of the yellow coloured species (λmax: 470 nm),whereas method B is based on oxidative coupling reaction used promethazine hydrochloride reagent and sodium hypochlorite oxidation agent in alkaline medium to form red colored measurable at 525 nm.The working conditions of both methods have been optimized. Regression analysis of Beer ’s law plots showed good correlation in the concentration ranges 5-65 and 2-50 μg ml-1 for methods A and B, respectively. The apparent molar absorptivity and Sandell sensitivity values are calculated to be 3.214x103and 1.028x104 l. mol-1 cm-1, and 0.0769 and 0.0267 μg cm-2,with LOD 0.552 μg ml- 1and 0.285μg ml-1, LOQ 1.840μg ml-1 and 0.942μg ml-1,respectively, for methods A and B. The methods were successfully applied to the determination of tinidazole in bulk drug and its formulations. Excipients used as additives in formulations did not interfere. Statistical treatment of the experimental results indicates that the accuracy and precision of the methods are analytically acceptable. The validity of the methods was evaluated by parallel determination by an established procedure, and by recovery studies


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