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Spectrophotometric Simultaneous Determination of Dapoxetine and Sildenafil in Combined Tablet Dosage Form by Absorbance Corrected Method. | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Spectrophotometric Simultaneous Determination of Dapoxetine and Sildenafil in Combined Tablet Dosage Form by Absorbance Corrected Method.

Author(s): Anuruddha P. Chabukswar,Bhanudas S. Kuchekar, Sonali L. Patil, Swagati A. Moon,Subhash G. Chate, Bharat D. Pagare.

One simple, economical, precise and accurate method is described for the simultaneous determination of Dapoxetine and Sildenafil in combined tablet dosage form. The method is Absorption Corrected Method. The amplitudes at 237.54 nm and 325.92 nm in the Absorption Corrected Method were selected to determine DPT and SL, respectively in combined formulation. The method was validated by following the analytical performance parameters suggested by the International Conference on Harmonization (ICH). All validation parameters were within the acceptable range. Under experimental conditions described, calibration curve, assay of tablets and recovery studies were performed. A critical evaluation of proposed method was performed by statistical analysis of data where slope, intercept, correlation coefficient is shown in Table (1). As per the ICH guidelines, the method validation parameters checked. Beer’s law is obeyed in the concentration range of 5-25 μg/ml for DPT and 8-40 μg/ml for SL by the given method.


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