Siddha was an ancient system of medicine which belongs to south India especially Tamilnadu. Vasanthakusumakara mathirai (VKM) was a herbomineral formulation used for bronchial asthma, tuberculosis, common cold, fever etc. To establish a medicine for worldwide it need to be standardize. This drug was standardized by various parameters like physio chemical, phyto chemical, biochemical analysis and High Performance Thin Layer Chromatography (HPTLC). In physio chemical analysis, the pH, ash value, loss on drying disintegration time given that this drug has alkaline pH with high purity and prepared in the Good Laboratory Practise in hygienic manner. The phyto chemical analysis established the presence of chemical constituents which has been helpful for its pharmacological action. The presence of acid and basic radicals has been tested in biochemical analysis which has the potent radicals used to promote the action of this drug. The fingerprint of this drug was scanned by HPTLC at different wavelength. For resolve the contamination, microbial load has been done by agar plating method which shows that bacterial and fungal load are within the limit. This will promote the drug for further evaluation in future.