In present research work , 2-aminobenzothiazole is condensed with different aromatic aldehydes in ethanol to yield corresponding Schiff bases. The Schiff bases were cyclised with chloroacetyl choride in triethyl amine to yield the corresponding 2-Azetidinones. The structures of synthesized compounds were confirmed by elemental and spectral analysis such as IR, NMR, Mass, GC-MS. The compounds were evaluated for their antimicrobial properties against microorganisms such as S. aureus , B. substilis., B megatherium, E. coli, S. typhie, P.aeruginosa.